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Ionizing radiation modulates vascular endothelial growth factor (VEGF) expression through multiple mitogen activated protein kinase dependent pathways

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Primary antibodies from Cell Signaling Technology were as followed: Akt (4691 T, 1:1000), Pho-Akt (Ser473) (4060 T, 1:1000), NF-κB (p65) (8242 T, 1:1000), Pho-NF-κB (Ser536) (3033 T, 1:1000), MAPK (p38) (8690 T, 1:1000), Pho-MAPK (Thr180/Tyr182) (4511 T, 1:1000), E-Cadherin (3195 s, 1:1000), N-Cadherin (13116 s, 1:500), and Vimentin (5741 s, 1:500)